Bioinformatic developments leading to the development of new leads and innovative diagnostics
Based on its over 18-year experience of working with computational experts and biological specialists, Acobiom’s bioinformatic platform has been dedicated to genomic and transcriptomic data management.This bioinformatic and biostatistical platform plays a leading role in the following developments:
- analyzing the results generated by new high throughput platforms, including:
- Sequence analysis,
- Analysis of gene expression,
- Analysis of regulation,
- Measuring biodiversity,
- integrating all these data into a network of interacting routes which provide the biotechnology industry and clinicians with the accurate targets or right information for curing patients.
Due to the fact that diseases and physiological adaptations are not linked to a single genetic modification occurring in a specific manner, but to complex routes associated with genetic networks, which interact with each other, Acobiom has developed bioinformatic tools to help researchers and physicians to:
- understand the complex interactions between the genetic networks,
- gather considerable amounts of closely related medical and biological data,
- provide a comprehensive overview of diseases through the data connection.
This comprehensive overview of disorders will enable to:
- improve treatments,
- reduce the cost of care through a more efficient treatment targeting strategy,
- contribute to the development of new drugs or new treatments for serious diseases with significant unmet medical needs.
Therefore, Acobiom’s bioinformatic platform representes a driving factor in computational sciences for managing the wealth of data that are to be generated in the medical revolution over the next decade.
Bioinformatics for gene expression analysis
Except for some particular cases which are now well-known, most recent medical improvements and studies have underlined that diseases and physiological adaptations are not linked to a single genetic modification (DNA mutation) occurring in a specific manner, but to complex routes associated with genetic networks, which interact with each other.
Understanding these complex interactions is the key challenge to be met in the medical field over the next decade. To reach this objective, scientists worldwide gathered an impressive amount of medical and biological data which need to be closely connected in order to provide a comprehensive overview of diseases. This comprehensive overview of medical disorders will enable to improve treatments within the field of Precision Medicine and will help to develop new drugs or treatments for diseases with significant unmet medical needs.
At the same time, high-performance computing applications increasingly adopted new architecture–based servers. Morever, new sequencing technologies made aslso huge progresses and provide today a considerable amount of data (BIG DATA) which have to be processed by a high-performance computer (HPC).
Acobiom provides private bioinformatic services dedicated to genomic analyses and to the implementation of these (Big) data in pathways and clinical routes.
Bioinformatics for sequencing data analysis
Regarding the expansion of high throughput sequencing technologies, generating sequencing data becomes very easy, but analyzing these BIG DATA becomes a serious and huge challenge.
Therefore, Acobiom developped specific bioinformatic programs and databases for analyzing the results generated by these high throughput sequencer systems, including sequence analysis, gene annotation, computational evolutionary biology, measuring biodiversity, analysis of regulation, analysis of protein expression, analysis of mutation in cancer, etc.
Within the quest for the $1,000 Human Genome, the bioinformatic platform of Acobiom will be a key tool for comparing each patient with known data regarding medical disorders.
Acobiom’s private bioinformatic platform is especially focused on genomic data management. This platform is based on a 18-year experience gained through the association of computational and biological experts.
Bioinformatic capabilities to treat omic BIG DATA
The ongoing development of molecular measurement technology provides a huge amount of data, the BIG DATA.
In two decades, scientists have gone from the sheet of paper for reading sequencing data to the receipt of a hard drive containing several Gigabits of Data, the Origin of Big Data. To analyze these Big Data, scientists have to switch their traditional easy-to-use tool or database to multiprocessors, multicores and parallelism platforms and programs. But these changes allow observing cellular systems at an increasing number of levels and in increasing details, which impose increasing demands on statistical methods and bioinformatic tools for the analysis and the management of this huge amount of data generated by these technologies.
Beyond the early stages of the simple commercial availabilities, Acobiom proposes specific softwares, programs and algorithms to treat and analyze omic Big Data obtained from NGS or qPCR systems.
Facing to the increasing demands on statistical methods and bioinformatic tools for the analysis and the management of the huge amounts of data, Acobiom adjusts tools and parameters to the specifications of its partners and their specific biological requests. It may concern RNA profiling (RNAseq, microRNA, etc.), DNA (full genome, HLA typing, targeting resequencing, ATACseq, etc.), a De novo Assembly, a SNPs or InDels detection, an Alignment analysis..
MaRS (Matrix of RNA-Seq) project
The aim of the MaRS research program is to collect and to allow the comparison of the NGS transcriptomic data produced by laboratories worldwide. MaRS is focused on the RNA‐Seq method, which reflects the expression of the genes in a specific condition.
MaRS contains 27,000 Human RNA‐Seq profiles generated by Next Generation Sequencing.
All these data have been collected and computed with a single method to allow their compilation in one matrix: MaRS. It represents a huge amount of data (#BigData) and requires the use of High Performance Computing (HPC) on the cluster Occigen (CINES): 120 To of compressed data downloaded and 1.2 million hours/core consumed.
The MaRS (Matrix of RNA-Seq) project has been granted by the SHAPE EU Program organized by the PRACE Initiative (www.prace-ri.eu/hpc-access/shape-programme/).
SHAPE, the SME HPC Adoption Programme in Europe, is a pan-European, PRACE-based programme supporting HPC adoption by SMEs. This Programme aims to raise awareness and equip European SMEs with the expertise necessary to take advantage of the innovation possibilities opened up by High Performance Computing (HPC), thus increasing their competitiveness.
MaRS contains Human RNA‐Seq profiles from different tissues. For instance, 1,100 RNA-Seq profiles concern Skin.
From these transcriptomic profiles, scientists can identify new RNA biomarkers involved in specific biological mechanisms or can also validate their RNA biomarkers identifyied from other sources.
A presentation of Skin data present in the MaRS database can be downloaded here: MaRS SKIN DATA .
During the European HPC Summit Week (May 16-17, 2017) in Barcelona Acobiom promoted the industrial program MARS (Matrix of RNAseq), as part of the PRACEdays17 – the fourth edition of the PRACE Scientific and Industrial Conference – under the motto “HPC for Innovation: when Science meets Industry”. The Acobiom’s MaRS database and its manager, Fabien Pierrat, have been awarded for best industrial presentation. To download this presentation: Mars PRACE Days Presentation 2017 and the poster: MaRS PRACE Days Poster 2017
Examples of Bioinformatics and Biostatistics treatments associated with transcriptomics analyses